rabbit myosin7a antibody  (Proteintech)

Journal: Nucleic Acids Research

Article Title: Fetal antisense oligonucleotide therapy for congenital deafness and vestibular dysfunction

doi: 10.1093/nar/gkaa194

Figure Lengend Snippet: ASO-29 enhances inner and outer hair cell survival. ( A ) Representative whole mount Myosin7a (Myo7a) confocal microscopic projections showing enhanced hair cell survival at 1.0, 2.0 and 3.0 mm from the tip of the cochlear apex (8–24 kHz inclusive) in ASO-29-treated mutants at P30. Distance from the apical tip was translated to frequencies using the cochlear place frequency map shown in . Scale bar: 20 μm. ( B ) Quantification of IHCs and OHCs ± SEM 0.5 to 4 mm from the tip of the cochlear apex (8–32 kHz inclusive). Untreated mutants were compared to the two ASO-29 treatment groups by two-way repeated measures ANOVA with Tukey's Multiple Comparison Test: * P < 0.05, ** P < 0.01, n = 5 per group. The number of outer hair cells in ASO-29 mutants and ASO-29 heterozygotes was significantly different only at 4 mm by two-way repeated measures ANOVA with Tukey's Multiple Comparison Test: † P < 0.05, n = 5 per group.

Article Snippet: A 1:100 dilution of rabbit Myosin7a (Proteus Biosciences Inc., Ramona, CA, USA) was applied in blocking solution overnight at 4°C with gentle agitation.

Techniques: Comparison

Journal: Cell Stress & Chaperones

Article Title: Upregulation of HSP60 expression in the postnatal rat cochlea and rats with drug-induced hearing loss

doi: 10.1007/s12192-018-0938-6

Figure Lengend Snippet: Establishment and validation of the drug-induced hearing loss model. a ABR thresholds of the experimental group increased after drug injection. ***p < 0.001. b Immunofluorescence staining demonstrated HCs loss in the experimental group. Myosin7a (green) was used as a HC marker. Scale bar = 20 μm

Article Snippet: The samples were then incubated with the following primary antibodies overnight at 4 °C: mouse anti-HSP60 monoclonal antibody (1:100, Millipore), rabbit anti-myosin7a monoclonal antibody (1:1000, Proteus Bioscience), and goat anti-SOX2 polyclonal antibody (1:100, Santa Cruz Biotechnology).

Techniques: Injection, Immunofluorescence, Staining, Marker

Journal: Cell Stress & Chaperones

Article Title: Upregulation of HSP60 expression in the postnatal rat cochlea and rats with drug-induced hearing loss

doi: 10.1007/s12192-018-0938-6

Figure Lengend Snippet: Upregulation of HSP60 expression in the cochlea after drug injury. a Immunofluorescence staining showed that HSP60 (green) expression was increased in the experimental group. Myosin7a (red) was used as a HC marker. Scale bar = 20 μm. b, c, and d qRT-PCR and Western blot analysis confirmed that the expression levels of Hsp60 gene and HSP60 protein in the experimental group were significantly higher than those in the control group ***p < 0.001

Article Snippet: The samples were then incubated with the following primary antibodies overnight at 4 °C: mouse anti-HSP60 monoclonal antibody (1:100, Millipore), rabbit anti-myosin7a monoclonal antibody (1:1000, Proteus Bioscience), and goat anti-SOX2 polyclonal antibody (1:100, Santa Cruz Biotechnology).

Techniques: Expressing, Immunofluorescence, Staining, Marker, Quantitative RT-PCR, Western Blot